Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory receptors in a teleost fish: typical CD28, elusive CTLA4.

doi: 10.4049/jimmunol.176.7.4191

Figure Lengend Snippet: FIGURE 8. Combined triggering of CD3 and huCD28/rbtCD28 in- duces IL-2 production. Jurkat CH7C17 transfectants expressing huCD28 wild-type (A14), huCD28/rbtCD28 receptor (2F5), huCD28/ rbtCTLA4 receptor (26H6) were incubated with ( ) or without (f) anti-human CD28 Ab for 30 min at 4°C. After washing, cells were deposited on anti-CD3-coated plates, and goat anti-mouse IgG1 Ab was added to cross-link human CD28 or chimeric receptors. Cells incubated only with goat anti-mouse IgG1 Ab were used as a control (). Su- pernatants were collected after 24 h at 37°C, and the IL-2 titer was determined by ELISA. IL-2 concentration is indicated in pg/10 ml. Asterisks indicate that the production of IL-2 by cells incubated using anti-human CD3-coated plates in the presence of anti-human CD28 Ab was significantly different from the production of IL-2 by cells solely plated on anti-human CD3-coated wells (two-tailed Mann-Whitney U test; , p 0.05, , p 0.01).

Article Snippet: Goat anti-mouse IgG1 Ab ( 1 chain specific; Southern Biotechnology Associates) was added (5 g/ ml) to cross-link human CD28.

Techniques: Expressing, Incubation, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Two Tailed Test, MANN-WHITNEY

Journal: eLife

Article Title: Glycan-based shaping of the microbiota during primate evolution

doi: 10.7554/eLife.67450

Figure Lengend Snippet:

Article Snippet: Antibody , Goat Anti-Mouse Polyclonal IgM, Human ads-HRP , Southern Biotech , Cat# 1020–05, RRID: AB_2794201 , 1:4000 (ELISA).

Techniques: Probiotics, Expressing, Bacteria, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Serial Dilution, Liposomes, Control, In Vivo, Staining, Software